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Cells were then permeabilized with 0.1% Triton-X-100 (Sigma-Aldrich) for 5 minutes, then incubated for 1 h with a saturation solution (10% of goat serum (Sigma-Aldrich), 3% of human serum (Sigma-Aldrich), 0.1% Triton-X-100 (Sigma-Aldrich), 1% BSA (Euromedex), 0.05% Tween 20 (Euromedex) in PBS

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The PI3K–AKT network at the interface of oncogenic signalling and cancer metabolism