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Add it to the water phase and keep processing temperatures below 40 °C (104°F)

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579 This staining combination provides two key insights: a mosaic pattern of SDH-positive but COX-negative fibers indicates an mtDNA defect involving COX, while a mosaic pattern of both SDH-positive and COX-positive fibers suggests an mtDNA defect involving NADH dehydrogenase (respiratory complex I) or cytochrome c reductase (respiratory complex III)