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The GLOW Blend should be stored refrigerated, unreconstituted, and protected from light, and is commonly reconstituted with bacteriostatic water prior to research use

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GSK3β inhibitor CHIR99021 (CHIR) activated Wnt/β-catenin to induce DE from hESCs combined with Nodal signaling activator activin A in RPMI + B27 medium (Raggi et al., 2022)