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Parent precursor masses of 124.0, 399.3, 385.1, and 665.1 Da and Q3 masses set to m/z 80.0, 250.1, 136.0, and 136.0 were used for the quantitation of NA, SAM, SAH, and NAD + , respectively

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Three-dimensional (3-D) model of vascular smooth muscle cells Rat vascular smooth muscle cells were maintained in high glucose DMEM supplemented with 10% fetal bovine serum