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One randomized controlled trial in 100 subjects with PCOS compared treatment with NAC (1,800 mg/day) to metformin (1,500 mg/day) for 24 weeks

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Sample Preparation The blood samples collected in the sterile heparinized tubes, centrifuged at 10,000 rpm for 15 min using refrigerated centrifugation machine (Sigma 2-16KL)