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The math is straightforward: Total Peptide Amount (mg) / Desired Concentration (mg/mL) = Volume of Diluent to Add (mL) For example, if you have a 10mg vial of tirzepatide and you want a final concentration of 5mg per 1mL, your calculation is: 10mg / 5mg/mL = 2mL So, you would need to add exactly 2mL of BAC water to the vial