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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RT–PCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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Studies have reported a 40% decrease in pro-inflammatory cytokine levels and a two-fold increase in antiviral immune pathway activation with hesperidin treatment in immune response models

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